Monday, August 23, 2010

Why certain organisms,cells are more suitable for the isolation of high molecular weight DNA?

I would look at it the other way. Why is it hard to isolate high molecular weight DNA from certain cells.





If the they are bacteria, or some other single celled organism, then it could be an unusually tough cell wall that resists simple DNA extractions. If it is a multicelluar organism, but real small, well then you have all the other parts of the organism around, and again it might be a just a question of the exact method of DNA extraction. For particular cell types or tissues you could find that there are a lot of enzymes present that could degrade the DNA and unless the extraction technique takes that into account, you could end up with smaller DNA pieces.





In general the techniques need to be modified depending on the organism or tissue that is being used.
  • eye make up
  • No comments:

    Post a Comment